Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10509 and 10510 are separated by 1519 nucleotides10510 and 10511 are separated by 347 nucleotides10511 and 10512 are separated by 289 nucleotides10512 and 10513 are separated by 476 nucleotides mRNA_2141: 10509 - SUPERFAMILY--SSF52047, at 175,857 to 177,524 10509 mRNA_2142: 10510 - Hypothetical Protein, at 179,044 to 180,564 10510 mRNA_2143: 10511 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SMART-A Receptor for Ubiquitination Targets-SM00256,SUPERFAMILY--SSF81383, at 180,912 to 181,824 10511 mRNA_2144: 10512 - Hypothetical Protein, at 182,114 to 183,256 10512 mRNA_2145: 10513 - HMMPfam-Protein of unknown function DUF89-PF01937,SUPERFAMILY--SSF111321, at 183,733 to 185,513 10513
Group Condition mRNA_2141 mRNA_2142 mRNA_2143 mRNA_2144 mRNA_2145
10509 10510 10511 10512 10513
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 -0.0 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.4 -0.1 +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.1 -0.2 -0.0
growth on ricinoleic acid YNB Glucose -0.1 +0.2 -0.3 -0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.1 -0.1 -0.1 +0.2
growth on oleic acid YNB Glucose -0.2 -0.3 +0.0 +0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.1 -0.3 -0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.1 -0.2 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 -0.0 +0.0 +0.0
auxotrophy Dropout Complete +0.2 +0.1 -0.2 -0.2 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.3 +0.1 +0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.2 -0.2 +0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 +0.0 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.2 +0.1 -0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.3 -0.1 +0.1 +0.3 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 +0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.4 +0.2 +0.4 +0.1
growth on ypd YPD +0.7 +0.2 -0.3 +0.2 +0.0
auxotrophy YNB Glucose +0.3 +0.1 -0.0 +0.0 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 -0.2 +0.3 +0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.3 +0.2 +0.2 +0.3
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