Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10513 and 10514 are separated by 248 nucleotides10514 and 10515 are separated by 98 nucleotides10515 and 10516 are separated by 120 nucleotides10516 and 10517 are separated by 124 nucleotides mRNA_2145: 10513 - HMMPfam-Protein of unknown function DUF89-PF01937,SUPERFAMILY--SSF111321, at 183,733 to 185,513 10513 mRNA_2146: 10514 - K15100 SLC25A1, CTP solute carrier family 25 (mitochondrial citrate transporter), member 1, at 185,762 to 187,254 10514 mRNA_2147: 10515 - K01719 hemD, UROS uroporphyrinogen-III synthase, at 187,353 to 188,300 10515 mRNA_2148: 10516 - KOG2490 Predicted membrane protein, at 188,421 to 191,027 10516 mRNA_2149: 10517 - K12948 SPCS3, SPC3 signal peptidase complex subunit 3, at 191,152 to 191,850 10517
Group Condition mRNA_2145 mRNA_2146 mRNA_2147 mRNA_2148 mRNA_2149
10513 10514 10515 10516 10517
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -1.7 N.D. +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.3 N.D. -1.1 N.D.
auxotrophy YNB Glucose +0.4 -0.7 N.D. -0.8 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.2 N.D. -0.9 N.D.
auxotrophy Dropout Complete +0.3 -0.2 N.D. -0.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.2 N.D. -0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.8 N.D. -0.0 N.D.
growth on ypd YPD +0.0 +0.1 N.D. -0.8 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.3 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.5 N.D. -0.5 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -1.1 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.3 -0.6 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.9 N.D. +0.7 N.D.
growth on oleic acid YNB Glucose +0.3 -0.6 N.D. +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.2 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.8 N.D. +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.7 N.D. +0.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.7 N.D. +0.9 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.4 N.D. +0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.4 +0.1 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.7 N.D. +0.7 N.D.
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