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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10514 and 10515 are separated by 98 nucleotides
10515 and 10516 are separated by 120 nucleotides
10516 and 10517 are separated by 124 nucleotides
10517 and 10518 are separated by 376 nucleotides
mRNA_2146: 10514 - K15100 SLC25A1, CTP solute carrier family 25 (mitochondrial citrate transporter), member 1, at 185,762 to 187,254
10514
mRNA_2147: 10515 - K01719 hemD, UROS uroporphyrinogen-III synthase, at 187,353 to 188,300
10515
mRNA_2148: 10516 - KOG2490 Predicted membrane protein, at 188,421 to 191,027
10516
mRNA_2149: 10517 - K12948 SPCS3, SPC3 signal peptidase complex subunit 3, at 191,152 to 191,850
10517
mRNA_2150: 10518 - Hypothetical Protein, at 192,227 to 192,680
10518
Group
Condition
mRNA
_2146
mRNA
_2147
mRNA
_2148
mRNA
_2149
mRNA
_2150
10514
10515
10516
10517
10518
auxotrophy
YNB Glucose
-0.7
N.D.
-0.8
N.D.
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
-1.7
N.D.
+0.3
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.3
N.D.
-1.1
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-0.2
N.D.
-0.9
N.D.
N.D.
auxotrophy
Dropout Complete
-0.2
N.D.
-0.9
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.5
N.D.
-0.5
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
-1.1
N.D.
+0.3
N.D.
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
N.D.
-0.6
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
-0.8
N.D.
-0.0
N.D.
N.D.
growth on ypd
YPD
+0.1
N.D.
-0.8
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.3
N.D.
-0.3
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
-0.6
N.D.
+0.1
N.D.
N.D.
growth on oleic acid
YNB Glucose
-0.6
N.D.
+0.2
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.9
N.D.
+0.7
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.2
N.D.
+0.1
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.7
N.D.
+0.7
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.8
N.D.
+0.7
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.4
N.D.
+0.4
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
+0.1
N.D.
-0.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.7
N.D.
+0.7
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.7
N.D.
+0.9
N.D.
N.D.
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