Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10515 and 10516 are separated by 120 nucleotides10516 and 10517 are separated by 124 nucleotides10517 and 10518 are separated by 376 nucleotides10518 and 10519 are separated by 233 nucleotides mRNA_2147: 10515 - K01719 hemD, UROS uroporphyrinogen-III synthase, at 187,353 to 188,300 10515 mRNA_2148: 10516 - KOG2490 Predicted membrane protein, at 188,421 to 191,027 10516 mRNA_2149: 10517 - K12948 SPCS3, SPC3 signal peptidase complex subunit 3, at 191,152 to 191,850 10517 mRNA_2150: 10518 - Hypothetical Protein, at 192,227 to 192,680 10518 mRNA_2151: 10519 - K03348 APC1 anaphase-promoting complex subunit 1, at 192,914 to 199,163 10519
Group Condition mRNA_2147 mRNA_2148 mRNA_2149 mRNA_2150 mRNA_2151
10515 10516 10517 10518 10519
growth on ypd YPD N.D. -0.8 N.D. N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine N.D. -0.9 N.D. N.D. -0.1
auxotrophy Dropout Complete N.D. -0.9 N.D. N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.1 N.D. N.D. +0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 N.D. N.D. -1.0
auxotrophy YNB Glucose N.D. -0.8 N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.6 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 N.D. N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.3 N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.7 N.D. N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 N.D. N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.7 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.7 N.D. N.D. +0.1
growth on oleic acid YNB Glucose N.D. +0.2 N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.9 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.7 N.D. N.D. +0.6
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