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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10516 and 10517 are separated by 124 nucleotides
10517 and 10518 are separated by 376 nucleotides
10518 and 10519 are separated by 233 nucleotides
10519 and 10520 are separated by 113 nucleotides
mRNA_2148: 10516 - KOG2490 Predicted membrane protein, at 188,421 to 191,027
10516
mRNA_2149: 10517 - K12948 SPCS3, SPC3 signal peptidase complex subunit 3, at 191,152 to 191,850
10517
mRNA_2150: 10518 - Hypothetical Protein, at 192,227 to 192,680
10518
mRNA_2151: 10519 - K03348 APC1 anaphase-promoting complex subunit 1, at 192,914 to 199,163
10519
mRNA_2152: 10520 - KOG2887 Membrane protein involved in ER to Golgi transport, at 199,277 to 200,467
10520
Group
Condition
mRNA
_2148
mRNA
_2149
mRNA
_2150
mRNA
_2151
mRNA
_2152
10516
10517
10518
10519
10520
ypd buoyancy
40 hrs No Buoyancy Separation
+0.3
N.D.
N.D.
-1.0
-1.1
auxotrophy
YNB Glucose 75mM Methionine
-0.9
N.D.
N.D.
-0.1
-0.2
growth on ypd
YPD
-0.8
N.D.
N.D.
-0.6
+0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-1.1
N.D.
N.D.
+0.4
-0.4
facs on lipid accumulation
Sorted No BODIPY Gate
-0.6
N.D.
N.D.
+0.1
-0.4
growth on oleic acid
YNB Oleic Acid
-0.1
N.D.
N.D.
+0.2
-1.0
auxotrophy
Dropout Complete
-0.9
N.D.
N.D.
+0.2
-0.0
auxotrophy
YNB Glucose
-0.8
N.D.
N.D.
+0.1
+0.0
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
N.D.
N.D.
-0.5
-0.2
ypd buoyancy
40 hrs Median Buoyancy
-0.3
N.D.
N.D.
+0.1
-0.3
growth on ricinoleic acid
YNB Glucose
+0.1
N.D.
N.D.
-0.4
+0.0
auxotrophy
YNB Glucose 75mM Arginine
-0.5
N.D.
N.D.
+0.3
-0.1
ypd buoyancy
40 hrs High Buoyancy
-0.0
N.D.
N.D.
+0.3
-0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.4
N.D.
N.D.
-0.1
+0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.7
N.D.
N.D.
-0.5
+0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
N.D.
N.D.
+0.2
+0.3
growth on oleic acid
YNB Glucose
+0.2
N.D.
N.D.
+0.7
+0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.7
N.D.
N.D.
+0.0
+0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.7
N.D.
N.D.
+0.1
+0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.9
N.D.
N.D.
+0.2
+0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.7
N.D.
N.D.
+0.6
+0.4
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