Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10516 and 10517 are separated by 124 nucleotides10517 and 10518 are separated by 376 nucleotides10518 and 10519 are separated by 233 nucleotides10519 and 10520 are separated by 113 nucleotides mRNA_2148: 10516 - KOG2490 Predicted membrane protein, at 188,421 to 191,027 10516 mRNA_2149: 10517 - K12948 SPCS3, SPC3 signal peptidase complex subunit 3, at 191,152 to 191,850 10517 mRNA_2150: 10518 - Hypothetical Protein, at 192,227 to 192,680 10518 mRNA_2151: 10519 - K03348 APC1 anaphase-promoting complex subunit 1, at 192,914 to 199,163 10519 mRNA_2152: 10520 - KOG2887 Membrane protein involved in ER to Golgi transport, at 199,277 to 200,467 10520
Group Condition mRNA_2148 mRNA_2149 mRNA_2150 mRNA_2151 mRNA_2152
10516 10517 10518 10519 10520
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -1.0 -1.1
auxotrophy YNB Glucose 75mM Methionine -0.9 N.D. N.D. -0.1 -0.2
growth on ypd YPD -0.8 N.D. N.D. -0.6 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 N.D. N.D. +0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. N.D. +0.1 -0.4
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +0.2 -1.0
auxotrophy Dropout Complete -0.9 N.D. N.D. +0.2 -0.0
auxotrophy YNB Glucose -0.8 N.D. N.D. +0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. -0.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. N.D. +0.1 -0.3
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.4 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. N.D. +0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. +0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 N.D. N.D. -0.5 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. N.D. +0.2 +0.3
growth on oleic acid YNB Glucose +0.2 N.D. N.D. +0.7 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. N.D. +0.0 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 N.D. N.D. +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 N.D. N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 N.D. N.D. +0.6 +0.4
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