Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10517 and 10518 are separated by 376 nucleotides10518 and 10519 are separated by 233 nucleotides10519 and 10520 are separated by 113 nucleotides10520 and 10521 are separated by 120 nucleotides mRNA_2149: 10517 - K12948 SPCS3, SPC3 signal peptidase complex subunit 3, at 191,152 to 191,850 10517 mRNA_2150: 10518 - Hypothetical Protein, at 192,227 to 192,680 10518 mRNA_2151: 10519 - K03348 APC1 anaphase-promoting complex subunit 1, at 192,914 to 199,163 10519 mRNA_2152: 10520 - KOG2887 Membrane protein involved in ER to Golgi transport, at 199,277 to 200,467 10520 mRNA_2153: 10521 - K14003 PREB, SEC12 prolactin regulatory element-binding protein, at 200,588 to 202,509 10521
Group Condition mRNA_2149 mRNA_2150 mRNA_2151 mRNA_2152 mRNA_2153
10517 10518 10519 10520 10521
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -1.0 -1.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 -1.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.5 -0.2 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.4 +0.0 N.D.
growth on ypd YPD N.D. N.D. -0.6 +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.5 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.4 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.3 -0.2 N.D.
auxotrophy YNB Glucose N.D. N.D. +0.1 +0.0 N.D.
auxotrophy Dropout Complete N.D. N.D. +0.2 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.3 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.0 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 +0.3 N.D.
growth on oleic acid YNB Glucose N.D. N.D. +0.7 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.6 +0.4 N.D.
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