Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10520 and 10521 are separated by 120 nucleotides10521 and 10522 are separated by 234 nucleotides10522 and 10523 are separated by 359 nucleotides10523 and 10524 are separated by 536 nucleotides mRNA_2152: 10520 - KOG2887 Membrane protein involved in ER to Golgi transport, at 199,277 to 200,467 10520 mRNA_2153: 10521 - K14003 PREB, SEC12 prolactin regulatory element-binding protein, at 200,588 to 202,509 10521 mRNA_2154: 10522 - KOG2689 Predicted ubiquitin regulatory protein, at 202,744 to 204,130 10522 mRNA_2155: 10523 - Hypothetical Protein, at 204,490 to 205,403 10523 mRNA_2156: 10524 - Hypothetical Protein, at 205,940 to 207,081 10524
Group Condition mRNA_2152 mRNA_2153 mRNA_2154 mRNA_2155 mRNA_2156
10520 10521 10522 10523 10524
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 N.D. -0.4 +0.3 -0.1
growth on oleic acid YNB Oleic Acid -1.0 N.D. -0.1 -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. +0.1 -0.4 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. -0.2 -0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.3 -0.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. +0.3 -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.3 -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.0 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. -0.0 -0.1 -0.1
growth on ypd YPD +0.3 N.D. +0.0 -0.2 +0.1
auxotrophy Dropout Complete -0.0 N.D. -0.2 +0.0 +0.5
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.2 +0.2 +0.5
growth on oleic acid YNB Glucose +0.0 N.D. +0.0 -0.1 +0.3
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.2 -0.2 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.1 -0.1 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. +0.1 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. +0.3 +0.0 +0.0
auxotrophy YNB Glucose +0.0 N.D. +0.1 +0.0 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.1 +0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.1 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. +0.2 +0.4 +0.3
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