Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10529 and 10530 are separated by 339 nucleotides10530 and 10531 are separated by 233 nucleotides10531 and 10532 are separated by 200 nucleotides10532 and 10533 are separated by 169 nucleotides mRNA_2161: 10529 - K03458 TC.NCS2 nucleobase-cation symporter-2, NCS2 family, at 220,965 to 223,272 10529 mRNA_2162: 10530 - K15027 EIF2D translation initiation factor 2D, at 223,612 to 225,989 10530 mRNA_2163: 10531 - K18468 VPS35 vacuolar protein sorting-associated protein 35, at 226,223 to 229,992 10531 mRNA_2164: 10532 - K10736 MCM10 minichromosome maintenance protein 10, at 230,193 to 233,259 10532 mRNA_2165: 10533 - Hypothetical Protein, at 233,429 to 233,973 10533
Group Condition mRNA_2161 mRNA_2162 mRNA_2163 mRNA_2164 mRNA_2165
10529 10530 10531 10532 10533
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.5 N.D. -1.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.2 N.D. -0.6 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.3 N.D. -0.7 -0.2
growth on oleic acid YNB Oleic Acid +0.0 -0.4 N.D. -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.7 N.D. -0.4 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 N.D. -0.4 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.1 N.D. -1.0 +0.2
growth on ricinoleic acid YNB Glucose +0.0 -0.4 N.D. -0.2 +0.0
auxotrophy Dropout Complete -0.1 -0.3 N.D. -0.3 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.1 N.D. -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 N.D. -0.6 -0.1
growth on ypd YPD +0.5 -0.3 N.D. -0.5 +0.2
auxotrophy YNB Glucose +0.3 -0.3 N.D. -0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.0 N.D. -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 N.D. +0.2 -0.0
growth on oleic acid YNB Glucose +0.1 -0.5 N.D. +0.5 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 N.D. +0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.5 N.D. -0.5 +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.2 N.D. +0.6 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.8 N.D. -0.0 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.5 N.D. -0.3 +0.5
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