Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10530 and 10531 are separated by 233 nucleotides10531 and 10532 are separated by 200 nucleotides10532 and 10533 are separated by 169 nucleotides10533 and 10534 are separated by 726 nucleotides mRNA_2162: 10530 - K15027 EIF2D translation initiation factor 2D, at 223,612 to 225,989 10530 mRNA_2163: 10531 - K18468 VPS35 vacuolar protein sorting-associated protein 35, at 226,223 to 229,992 10531 mRNA_2164: 10532 - K10736 MCM10 minichromosome maintenance protein 10, at 230,193 to 233,259 10532 mRNA_2165: 10533 - Hypothetical Protein, at 233,429 to 233,973 10533 mRNA_2166: 10534 - Hypothetical Protein, at 234,700 to 236,014 10534
Group Condition mRNA_2162 mRNA_2163 mRNA_2164 mRNA_2165 mRNA_2166
10530 10531 10532 10533 10534
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. -1.2 -0.5 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.6 -0.7 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. -0.7 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -1.0 +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.7 N.D. -0.4 +0.1 -0.2
growth on oleic acid YNB Oleic Acid -0.4 N.D. -0.1 -0.4 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.4 -0.2 -0.0
growth on ypd YPD -0.3 N.D. -0.5 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.6 -0.1 -0.0
growth on ricinoleic acid YNB Glucose -0.4 N.D. -0.2 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.4 +0.0 +0.0
auxotrophy YNB Glucose -0.3 N.D. -0.3 +0.1 -0.0
auxotrophy Dropout Complete -0.3 N.D. -0.3 +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.2 +0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.2 -0.0 +0.1
growth on oleic acid YNB Glucose -0.5 N.D. +0.5 +0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.5 N.D. -0.5 +0.4 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.2 +0.2 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.6 +0.3 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. -0.3 +0.5 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 N.D. -0.0 +0.3 +0.1
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