Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10537 and 10538 are separated by 84 nucleotides10538 and 10539 are separated by 419 nucleotides10539 and 10540 are separated by 385 nucleotides10540 and 10541 are separated by 524 nucleotides mRNA_2169: 10537 - Hypothetical Protein, at 239,219 to 240,668 10537 mRNA_2170: 10538 - K20294 COG7 conserved oligomeric Golgi complex subunit 7, at 240,753 to 243,794 10538 mRNA_2171: 10539 - K06877 K06877 DEAD/DEAH box helicase domain-containing protein, at 244,214 to 249,218 10539 mRNA_2172: 10540 - K00968 PCYT1 choline-phosphate cytidylyltransferase, at 249,604 to 252,159 10540 mRNA_2173: 10541 - K03950 NDUFA6 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 6, at 252,684 to 253,574 10541
Group Condition mRNA_2169 mRNA_2170 mRNA_2171 mRNA_2172 mRNA_2173
10537 10538 10539 10540 10541
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 -0.2 +0.2 -2.0
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.1 +0.1 +0.3 -1.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.6 +0.1 +0.3 -2.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 -0.1 +0.3 -1.1
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.5 -0.1 +0.3 -1.5
auxotrophy Dropout Complete -0.2 +0.4 -0.2 +0.4 -1.3
growth on ricinoleic acid YNB Glucose +0.0 +0.3 -0.1 +0.4 -1.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.4 -0.2 +0.3 -1.4
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.1 +0.1 +0.1 -1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.5 -0.1 +0.4 -1.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.3 -0.2 +0.4 -0.4
growth on ypd YPD +0.1 +0.1 -0.1 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.7 -0.0 -0.4 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.8 -0.0 +0.6 -1.2
growth on oleic acid YNB Glucose -0.1 +0.3 -0.2 +0.4 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.2 -0.1 +0.5 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.4 -0.1 +0.5 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.3 +0.1 +0.8 -0.5
auxotrophy YNB Glucose +0.1 +0.5 -0.2 +0.7 -0.7
growth on oleic acid YNB Oleic Acid +0.1 +0.4 -0.3 +0.4 +0.8
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.2 -0.0 +1.0 +1.1
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