Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10539 and 10540 are separated by 385 nucleotides10540 and 10541 are separated by 524 nucleotides10541 and 10542 are separated by 452 nucleotides10542 and 10543 are separated by 293 nucleotides mRNA_2171: 10539 - K06877 K06877 DEAD/DEAH box helicase domain-containing protein, at 244,214 to 249,218 10539 mRNA_2172: 10540 - K00968 PCYT1 choline-phosphate cytidylyltransferase, at 249,604 to 252,159 10540 mRNA_2173: 10541 - K03950 NDUFA6 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 6, at 252,684 to 253,574 10541 mRNA_2174: 10542 - K14009 BCAP31, BAP31 B-cell receptor-associated protein 31, at 254,027 to 255,180 10542 mRNA_2175: 10543 - HMMPfam-Mus7/MMS22 family-PF09462, at 255,474 to 261,372 10543
Group Condition mRNA_2171 mRNA_2172 mRNA_2173 mRNA_2174 mRNA_2175
10539 10540 10541 10542 10543
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.3 -2.4 -0.3 -0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.3 -1.9 +0.0 -1.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.3 -1.4 -0.3 -0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 -1.2 -0.0 -1.1
auxotrophy Dropout Complete -0.2 +0.4 -1.3 +0.1 -1.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.8 -0.5 -0.1 -2.0
growth on ricinoleic acid YNB Glucose -0.1 +0.4 -1.4 -0.1 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 -2.0 +0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.3 -1.5 +0.1 +0.1
growth on ypd YPD -0.1 -0.2 +0.0 +0.0 -0.6
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.4 -0.4 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.4 -0.3 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.5 -0.5 +0.1 -0.5
growth on oleic acid YNB Glucose -0.2 +0.4 -0.3 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.4 -1.0 +0.0 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.3 -1.1 +0.2 +0.6
auxotrophy YNB Glucose -0.2 +0.7 -0.7 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.6 -1.2 +0.3 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.5 -0.3 -0.1 +0.3
growth on oleic acid YNB Oleic Acid -0.3 +0.4 +0.8 -0.4 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 +1.0 +1.1 -0.0 +0.3
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