Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10551 and 10552 are separated by 216 nucleotides10552 and 10553 are separated by 768 nucleotides10553 and 10554 are separated by 1069 nucleotides10554 and 10555 are separated by 371 nucleotides mRNA_2183: 10551 - K00059 fabG 3-oxoacyl-[acyl-carrier protein] reductase, at 284,423 to 286,019 10551 mRNA_2184: 10552 - Hypothetical Protein, at 286,236 to 287,917 10552 mRNA_2185: 10553 - HMMPfam-Copper fist DNA binding domain-PF00649,PRINTS-Copper fist domain signature-PR00617,ProSiteProfiles-Copper-fist DNA-binding domain profile.-PS50073,SMART-Copper-Fist-SM00412,SMART--SM01090,SUPERFAMILY--SSF57879, at 288,686 to 288,844 10553 mRNA_2186: 10554 - K06316 RFT1 oligosaccharide translocation protein RFT1, at 289,914 to 291,585 10554 mRNA_2187: 10555 - K09602 OTUB1 ubiquitin thioesterase protein OTUB1, at 291,957 to 293,217 10555
Group Condition mRNA_2183 mRNA_2184 mRNA_2185 mRNA_2186 mRNA_2187
10551 10552 10553 10554 10555
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.1 -1.0 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.4 -0.8 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.3 -0.4 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.1 -0.6 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.6 +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.1 -0.4 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.2 -0.8 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.1 -0.5 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.3 -0.8 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.2 -0.3 N.D. -0.3
growth on oleic acid YNB Oleic Acid -0.1 +0.0 +0.6 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.2 +0.1 -0.3 N.D. +0.4
growth on oleic acid YNB Glucose -0.1 -0.1 +0.8 N.D. -0.1
growth on ypd YPD +0.1 +0.1 +0.8 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.4 +1.7 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.2 +1.0 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.2 +1.5 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.1 +0.9 N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 +0.0 +1.1 N.D. +0.3
auxotrophy YNB Glucose +0.4 -0.2 +1.8 N.D. -0.1
auxotrophy Dropout Complete +0.1 -0.2 +2.3 N.D. -0.0
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