Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10554 and 10555 are separated by 371 nucleotides10555 and 10556 are separated by 941 nucleotides10556 and 10557 are separated by 1260 nucleotides10557 and 10558 are separated by 1468 nucleotides mRNA_2186: 10554 - K06316 RFT1 oligosaccharide translocation protein RFT1, at 289,914 to 291,585 10554 mRNA_2187: 10555 - K09602 OTUB1 ubiquitin thioesterase protein OTUB1, at 291,957 to 293,217 10555 mRNA_2188: 10556 - Hypothetical Protein, at 294,159 to 294,788 10556 mRNA_2189: 10557 - K11236 CDC24 cell division control protein 24, at 296,049 to 299,793 10557 mRNA_2190: 10558 - K00059 fabG 3-oxoacyl-[acyl-carrier protein] reductase, at 301,262 to 303,174 10558
Group Condition mRNA_2186 mRNA_2187 mRNA_2188 mRNA_2189 mRNA_2190
10554 10555 10556 10557 10558
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -1.0 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.4 N.D. -0.5 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 N.D. +0.2 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. +0.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. +0.4 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. +0.5 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. +1.0 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 N.D. +1.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 N.D. +0.9 +0.1
growth on oleic acid YNB Glucose N.D. -0.1 N.D. +0.8 +0.1
growth on ypd YPD N.D. -0.3 N.D. +0.8 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. +1.2 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. +1.2 +0.1
auxotrophy Dropout Complete N.D. -0.0 N.D. +1.2 -0.0
auxotrophy YNB Glucose N.D. -0.1 N.D. +1.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 N.D. +1.0 -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.4 N.D. +1.0 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. +2.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 N.D. +1.8 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. +2.9 +0.5
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