Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10559 and 10560 are separated by 220 nucleotides10560 and 10561 are separated by 148 nucleotides10561 and 10562 are separated by 487 nucleotides10562 and 10563 are separated by 1358 nucleotides mRNA_2191: 10559 - ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048, at 304,016 to 306,697 10559 mRNA_2192: 10560 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 306,918 to 310,071 10560 mRNA_2193: 10561 - HMMPfam-Alpha/beta hydrolase family-PF12697,SUPERFAMILY--SSF53474, at 310,220 to 312,834 10561 mRNA_2194: 10562 - BLAST ER to Golgi transport-related protein, putative [Cryp..., at 313,322 to 318,107 10562 mRNA_2195: 10563 - HMMPfam-Subtilase family-PF00082,HMMPfam-PA domain-PF02225,HMMPfam-Fn3-like domain (DUF1034)-PF06280,PRINTS-Subtilisin serine protease family (S8) signature-PR00723,ProSitePatterns-Serine proteases, subtilase family, aspartic acid active site.-PS00136,ProSitePatterns-Serine proteases, subtilase family, histidine active site.-PS00137,SUPERFAMILY--SSF52025,SUPERFAMILY--SSF52743, at 319,466 to 323,008 10563
Group Condition mRNA_2191 mRNA_2192 mRNA_2193 mRNA_2194 mRNA_2195
10559 10560 10561 10562 10563
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.3 -0.2 -0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 +0.2 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 -0.2 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.1 +0.3 -0.0 -0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.1 +0.2 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.1 +0.4 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 -0.0 -0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.5 -0.4 +0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.1 +0.3 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.1 -0.1 +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 +0.3 +0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 +0.0 +0.0 +0.0
growth on oleic acid YNB Glucose -0.0 -0.2 +0.2 +0.1 +0.2
growth on ricinoleic acid YNB Glucose -0.2 +0.2 +0.2 +0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.1 -0.1 +0.0 +0.1
auxotrophy Dropout Complete +0.1 -0.2 +0.5 -0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.2 +0.2 -0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 +0.3 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.1 +0.6 -0.1 +0.1
auxotrophy YNB Glucose +0.1 -0.2 +0.7 -0.1 +0.1
growth on ypd YPD +0.1 -0.1 +0.6 +0.0 +0.0
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