Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10568 and 10569 are separated by 236 nucleotides10569 and 10570 are separated by 318 nucleotides10570 and 10571 are separated by 321 nucleotides10571 and 10572 are separated by 267 nucleotides mRNA_2200: 10568 - K11423 SETD2, SET2 histone-lysine N-methyltransferase SETD2, at 335,567 to 339,379 10568 mRNA_2201: 10569 - K03005 RPA49, POLR1E DNA-directed RNA polymerase I subunit RPA49, at 339,616 to 341,199 10569 mRNA_2202: 10570 - K00365 uaZ urate oxidase, at 341,518 to 342,881 10570 mRNA_2203: 10571 - K13339 PEX6, PXAAA1 peroxin-6, at 343,203 to 347,570 10571 mRNA_2204: 10572 - K03786 aroQ, qutE 3-dehydroquinate dehydratase II, at 347,838 to 348,942 10572
Group Condition mRNA_2200 mRNA_2201 mRNA_2202 mRNA_2203 mRNA_2204
10568 10569 10570 10571 10572
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 -0.5 -0.0 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.1 -0.1 -0.0 N.D. -0.1
growth on ypd YPD -0.4 -0.4 +0.2 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy -0.7 -0.6 +0.3 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.8 -0.1 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.1 -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 +0.8 -0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.1 +0.1 N.D. -0.2
growth on oleic acid YNB Glucose -0.1 -0.2 +0.3 N.D. -0.0
growth on oleic acid YNB Oleic Acid -0.3 +0.2 +0.2 N.D. -0.0
auxotrophy Dropout Complete -0.3 -0.2 +0.4 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 +0.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.1 -0.2 +0.3 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 +0.2 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.2 +0.3 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 +0.4 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.6 +0.4 N.D. -0.0
auxotrophy YNB Glucose +0.2 +0.0 +0.5 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.1 +0.2 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +0.1 +0.3 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.8 +0.9 N.D. -0.2
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