Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10569 and 10570 are separated by 318 nucleotides10570 and 10571 are separated by 321 nucleotides10571 and 10572 are separated by 267 nucleotides10572 and 10573 are separated by 496 nucleotides mRNA_2201: 10569 - K03005 RPA49, POLR1E DNA-directed RNA polymerase I subunit RPA49, at 339,616 to 341,199 10569 mRNA_2202: 10570 - K00365 uaZ urate oxidase, at 341,518 to 342,881 10570 mRNA_2203: 10571 - K13339 PEX6, PXAAA1 peroxin-6, at 343,203 to 347,570 10571 mRNA_2204: 10572 - K03786 aroQ, qutE 3-dehydroquinate dehydratase II, at 347,838 to 348,942 10572 mRNA_2205: 10573 - KOG0381 HMG box-containing protein, at 349,439 to 350,135 10573
Group Condition mRNA_2201 mRNA_2202 mRNA_2203 mRNA_2204 mRNA_2205
10569 10570 10571 10572 10573
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.0 N.D. -0.5 N.D.
growth on ypd YPD -0.4 +0.2 N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.8 -0.1 N.D. +0.5 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.6 +0.3 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.0 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose -0.2 +0.3 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.3 N.D. +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 -0.3 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.2 +0.3 N.D. +0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.2 N.D. -0.0 N.D.
auxotrophy Dropout Complete -0.2 +0.4 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.4 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.2 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.3 N.D. +0.3 N.D.
auxotrophy YNB Glucose +0.0 +0.5 N.D. +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.6 +0.4 N.D. -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 +0.9 N.D. -0.2 N.D.
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