Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10572 and 10573 are separated by 496 nucleotides10573 and 10574 are separated by 705 nucleotides10574 and 10575 are separated by 277 nucleotides10575 and 10576 are separated by 238 nucleotides mRNA_2204: 10572 - K03786 aroQ, qutE 3-dehydroquinate dehydratase II, at 347,838 to 348,942 10572 mRNA_2205: 10573 - KOG0381 HMG box-containing protein, at 349,439 to 350,135 10573 mRNA_2206: 10574 - K20465 OSBPL9_10_11, ORP9_10_11 oxysterol-binding protein-related protein 9/10/11, at 350,841 to 352,829 10574 mRNA_2207: 10575 - K08284 MID1 calcium channel MID1, at 353,107 to 355,052 10575 mRNA_2208: 10576 - K13621 BTA1 betaine lipid synthase, at 355,291 to 358,512 10576
Group Condition mRNA_2204 mRNA_2205 mRNA_2206 mRNA_2207 mRNA_2208
10572 10573 10574 10575 10576
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.2 -0.5 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.1 -0.3 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. +0.1 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.1 -0.2 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.1 -0.1 -0.3
growth on oleic acid YNB Glucose -0.0 N.D. -0.2 +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. -0.0 -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.1 -0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.1 -0.1 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.1 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.0 -0.1 -0.3
growth on ypd YPD -0.4 N.D. -0.1 +0.2 +0.2
auxotrophy Dropout Complete +0.2 N.D. +0.2 -0.0 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.1 -0.0 -0.0
growth on oleic acid YNB Oleic Acid -0.0 N.D. -0.2 +0.4 -0.1
auxotrophy YNB Glucose +0.3 N.D. +0.1 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. +0.4 +0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.1 +0.5 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.3 +0.4 +0.2
growth on ricinoleic acid YNB Glucose +0.3 N.D. +0.1 +0.2 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. +0.2 +0.5 +0.1
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