Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10574 and 10575 are separated by 277 nucleotides10575 and 10576 are separated by 238 nucleotides10576 and 10577 are separated by 678 nucleotides10577 and 10578 are separated by 292 nucleotides mRNA_2206: 10574 - K20465 OSBPL9_10_11, ORP9_10_11 oxysterol-binding protein-related protein 9/10/11, at 350,841 to 352,829 10574 mRNA_2207: 10575 - K08284 MID1 calcium channel MID1, at 353,107 to 355,052 10575 mRNA_2208: 10576 - K13621 BTA1 betaine lipid synthase, at 355,291 to 358,512 10576 mRNA_2209: 10577 - K14157 AASS alpha-aminoadipic semialdehyde synthase, at 359,191 to 363,010 10577 mRNA_2210: 10578 - K20407 MIOS, MIO WD repeat-containing protein mio, at 363,303 to 367,211 10578
Group Condition mRNA_2206 mRNA_2207 mRNA_2208 mRNA_2209 mRNA_2210
10574 10575 10576 10577 10578
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.5 -0.1 -0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 -0.3 -0.4 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.1 -0.3 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.3 -0.1 -0.2 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 -0.4 -0.5 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 -0.3 +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.1 -0.0 +0.3 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 -0.1 +0.2 -0.0
growth on oleic acid YNB Glucose -0.2 +0.0 -0.1 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.2 -0.2 +0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.0 -0.0 +0.1 -0.1
growth on oleic acid YNB Oleic Acid -0.2 +0.4 -0.1 -0.2 +0.2
auxotrophy YNB Glucose +0.1 -0.1 -0.1 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.0 -0.2 +0.1 +0.2
auxotrophy Dropout Complete +0.2 -0.0 -0.3 +0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.0 -0.1 -0.0 +0.1
growth on ypd YPD -0.1 +0.2 +0.2 +0.0 +0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.2 +0.2 -0.3 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.5 -0.0 -0.0 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.4 +0.2 -0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.5 +0.1 +0.2 +0.0
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