Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10575 and 10576 are separated by 238 nucleotides10576 and 10577 are separated by 678 nucleotides10577 and 10578 are separated by 292 nucleotides10578 and 10579 are separated by 344 nucleotides mRNA_2207: 10575 - K08284 MID1 calcium channel MID1, at 353,107 to 355,052 10575 mRNA_2208: 10576 - K13621 BTA1 betaine lipid synthase, at 355,291 to 358,512 10576 mRNA_2209: 10577 - K14157 AASS alpha-aminoadipic semialdehyde synthase, at 359,191 to 363,010 10577 mRNA_2210: 10578 - K20407 MIOS, MIO WD repeat-containing protein mio, at 363,303 to 367,211 10578 mRNA_2211: 10579 - K09486 HYOU1 hypoxia up-regulated 1, at 367,556 to 370,614 10579
Group Condition mRNA_2207 mRNA_2208 mRNA_2209 mRNA_2210 mRNA_2211
10575 10576 10577 10578 10579
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.0 +0.3 -0.4 -2.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.3 -0.4 -0.0 -1.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.4 -0.5 +0.2 -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.1 -0.6 +0.1 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.1 -0.2 +0.0 -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 +0.2 -0.0 -1.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.3 +0.0 -0.1 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.2 +0.1 +0.2 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.3 -0.3 -0.0 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 -0.0 +0.1 -0.5
growth on oleic acid YNB Glucose +0.0 -0.1 -0.1 +0.2 -0.5
growth on oleic acid YNB Oleic Acid +0.4 -0.1 -0.2 +0.2 -0.7
auxotrophy Dropout Complete -0.0 -0.3 +0.3 +0.2 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.0 +0.1 -0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.2 +0.2 -0.3 +0.5 -0.5
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.2 +0.1 +0.0 +0.6
auxotrophy YNB Glucose -0.1 -0.1 +0.2 +0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.2 -0.2 +0.6 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.0 -0.0 +0.5 +0.2
growth on ypd YPD +0.2 +0.2 +0.0 +0.2 +0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.5 +0.1 +0.2 +0.0 +0.7
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