Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10577 and 10578 are separated by 292 nucleotides10578 and 10579 are separated by 344 nucleotides10579 and 10580 are separated by 665 nucleotides10580 and 10581 are separated by 336 nucleotides mRNA_2209: 10577 - K14157 AASS alpha-aminoadipic semialdehyde synthase, at 359,191 to 363,010 10577 mRNA_2210: 10578 - K20407 MIOS, MIO WD repeat-containing protein mio, at 363,303 to 367,211 10578 mRNA_2211: 10579 - K09486 HYOU1 hypoxia up-regulated 1, at 367,556 to 370,614 10579 mRNA_2212: 10580 - K04441 P38 p38 MAP kinase, at 371,280 to 373,458 10580 mRNA_2213: 10581 - Hypothetical Protein, at 373,795 to 375,886 10581
Group Condition mRNA_2209 mRNA_2210 mRNA_2211 mRNA_2212 mRNA_2213
10577 10578 10579 10580 10581
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.1 -0.5 -3.4 -0.2
growth on ricinoleic acid YNB Glucose -0.3 +0.5 -0.5 -3.1 +0.1
auxotrophy Dropout Complete +0.3 +0.2 -0.6 -2.9 -0.2
auxotrophy YNB Glucose +0.2 +0.2 +0.3 -3.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.0 -1.1 -0.8 +0.1
growth on oleic acid YNB Glucose -0.1 +0.2 -0.5 -1.8 -0.0
growth on oleic acid YNB Oleic Acid -0.2 +0.2 -0.7 -1.6 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 +0.1 -0.6 -0.8 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.4 -2.3 +0.4 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.6 -0.6 -2.2 +0.6
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.0 +0.6 -2.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.5 +0.2 -1.0 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.0 -0.7 +0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.5 +0.2 -2.1 +0.8
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.0 -1.0 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.0 +0.2 +0.0 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.2 -0.8 +0.5 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 -0.5 +0.9 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.0 +0.7 -0.9 +0.6
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 -0.1 +0.9 +0.1
growth on ypd YPD +0.0 +0.2 +0.7 -0.0 +0.0
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