Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10579 and 10580 are separated by 665 nucleotides10580 and 10581 are separated by 336 nucleotides10581 and 10582 are separated by 676 nucleotides10582 and 10583 are separated by 138 nucleotides mRNA_2211: 10579 - K09486 HYOU1 hypoxia up-regulated 1, at 367,556 to 370,614 10579 mRNA_2212: 10580 - K04441 P38 p38 MAP kinase, at 371,280 to 373,458 10580 mRNA_2213: 10581 - Hypothetical Protein, at 373,795 to 375,886 10581 mRNA_2214: 10582 - K06970 rlmF 23S rRNA (adenine1618-N6)-methyltransferase, at 376,563 to 378,868 10582 mRNA_2215: 10583 - K01737 queD, ptpS, PTS 6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase, at 379,007 to 379,807 10583
Group Condition mRNA_2211 mRNA_2212 mRNA_2213 mRNA_2214 mRNA_2215
10579 10580 10581 10582 10583
auxotrophy Dropout Complete -0.6 -2.9 -0.2 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.5 -3.4 -0.2 N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.5 -3.1 +0.1 N.D. -0.1
auxotrophy YNB Glucose +0.3 -3.4 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.1 -0.8 +0.1 N.D. -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 -2.2 +0.6 N.D. -0.3
growth on oleic acid YNB Glucose -0.5 -1.8 -0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -0.8 -0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -2.3 +0.4 +0.3 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.7 -1.6 +0.3 N.D. +0.6
auxotrophy YNB Glucose 75mM Arginine +0.6 -2.0 +0.0 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -2.1 +0.8 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 +0.1 -0.2 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate -1.0 +0.1 +0.1 N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 +0.5 +0.2 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -1.0 +0.1 +0.4 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.0 +0.2 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.7 -0.9 +0.6 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.5 +0.9 +0.1 N.D. +0.1
growth on ypd YPD +0.7 -0.0 +0.0 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.9 +0.1 N.D. -0.1
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