Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10592 and 10593 are separated by 606 nucleotides10593 and 10594 are separated by 586 nucleotides10594 and 10595 are separated by 123 nucleotides10595 and 10596 are separated by 131 nucleotides mRNA_2224: 10592 - KOG2653 6-phosphogluconate dehydrogenase, at 400,569 to 403,367 10592 mRNA_2225: 10593 - Hypothetical Protein, at 403,974 to 404,899 10593 mRNA_2226: 10594 - SMART-SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains-SM00717, at 405,486 to 409,218 10594 mRNA_2227: 10595 - K12392 AP1B1 AP-1 complex subunit beta-1, at 409,342 to 412,255 10595 mRNA_2228: 10596 - K14191 DIM1 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase, at 412,387 to 413,949 10596
Group Condition mRNA_2224 mRNA_2225 mRNA_2226 mRNA_2227 mRNA_2228
10592 10593 10594 10595 10596
facs on lipid accumulation Sorted High BODIPY Gate -0.6 +0.0 -0.0 -2.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.1 -0.8 -1.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.1 -0.1 -1.2 N.D.
growth on oleic acid YNB Glucose +0.1 -0.5 -0.1 -0.7 N.D.
growth on oleic acid YNB Oleic Acid -0.1 -0.2 +0.2 -0.9 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.0 -0.1 -0.9 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.0 -0.1 -0.5 N.D.
auxotrophy Dropout Complete +0.2 +0.0 +0.0 -0.7 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.1 -0.2 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.3 -1.2 +1.7 N.D.
auxotrophy YNB Glucose +0.2 +0.2 +0.2 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.1 +0.3 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.3 +0.3 +0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.2 -0.3 -0.4 +1.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.0 +0.2 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.3 +0.3 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.2 +0.1 +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.5 -0.3 +1.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.0 +0.4 +0.2 N.D.
growth on ypd YPD +0.0 -0.0 -0.2 +1.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.1 +0.5 +0.4 N.D.
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