Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10595 and 10596 are separated by 131 nucleotides10596 and 10597 are separated by 2624 nucleotides10597 and 10598 are separated by 679 nucleotides10598 and 10599 are separated by 345 nucleotides mRNA_2227: 10595 - K12392 AP1B1 AP-1 complex subunit beta-1, at 409,342 to 412,255 10595 mRNA_2228: 10596 - K14191 DIM1 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase, at 412,387 to 413,949 10596 mRNA_2229: 10597 - HMMPfam-Hsp70 protein-PF00012,PRINTS-70kDa heat shock protein signature-PR00301,ProSitePatterns-Heat shock hsp70 proteins family signature 2.-PS00329,ProSitePatterns-Heat shock hsp70 proteins family signature 3.-PS01036,SUPERFAMILY--SSF100934,SUPERFAMILY--SSF53067, at 416,574 to 419,223 10597 mRNA_2230: 10598 - HMMPfam-Protein kinase domain-PF00069,ProSitePatterns-Serine/Threonine protein kinases active-site signature.-PS00108,ProSiteProfiles-Protein kinase domain profile.-PS50011,SMART-Serine/Threonine protein kinases, catalytic domain-SM00220,SUPERFAMILY--SSF56112, at 419,903 to 421,996 10598 mRNA_2231: 10599 - Hypothetical Protein, at 422,342 to 422,815 10599
Group Condition mRNA_2227 mRNA_2228 mRNA_2229 mRNA_2230 mRNA_2231
10595 10596 10597 10598 10599
auxotrophy YNB Glucose -0.6 N.D. -0.4 -1.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -2.2 N.D. +0.0 +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -1.2 N.D. +0.1 -0.8 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.5 N.D. -0.6 +0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. -0.7 -0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.9 N.D. -0.1 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.4 -1.2 N.D.
growth on oleic acid YNB Glucose -0.7 N.D. -0.2 -0.4 N.D.
auxotrophy Dropout Complete -0.7 N.D. -0.4 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.3 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.9 N.D. +0.1 +0.8 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -0.1 +0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 N.D. -0.4 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.3 +1.0 N.D.
growth on ricinoleic acid YNB Glucose +1.1 N.D. +0.6 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. -0.4 +1.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.1 +1.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. -0.1 +1.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +1.7 N.D. +0.4 -0.4 N.D.
growth on ypd YPD +1.2 N.D. -0.1 +0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +1.7 N.D. +0.3 +0.5 N.D.
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