Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10596 and 10597 are separated by 2624 nucleotides10597 and 10598 are separated by 679 nucleotides10598 and 10599 are separated by 345 nucleotides10599 and 10600 are separated by 1811 nucleotides mRNA_2228: 10596 - K14191 DIM1 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase, at 412,387 to 413,949 10596 mRNA_2229: 10597 - HMMPfam-Hsp70 protein-PF00012,PRINTS-70kDa heat shock protein signature-PR00301,ProSitePatterns-Heat shock hsp70 proteins family signature 2.-PS00329,ProSitePatterns-Heat shock hsp70 proteins family signature 3.-PS01036,SUPERFAMILY--SSF100934,SUPERFAMILY--SSF53067, at 416,574 to 419,223 10597 mRNA_2230: 10598 - HMMPfam-Protein kinase domain-PF00069,ProSitePatterns-Serine/Threonine protein kinases active-site signature.-PS00108,ProSiteProfiles-Protein kinase domain profile.-PS50011,SMART-Serine/Threonine protein kinases, catalytic domain-SM00220,SUPERFAMILY--SSF56112, at 419,903 to 421,996 10598 mRNA_2231: 10599 - Hypothetical Protein, at 422,342 to 422,815 10599 mRNA_2232: 10600 - Hypothetical Protein, at 424,627 to 425,038 10600
Group Condition mRNA_2228 mRNA_2229 mRNA_2230 mRNA_2231 mRNA_2232
10596 10597 10598 10599 10600
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.8 N.D. -0.7
auxotrophy YNB Glucose N.D. -0.4 -1.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -1.2 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 +1.0 N.D. -1.6
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.5 N.D. -0.1
auxotrophy Dropout Complete N.D. -0.4 -0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.7 -0.3 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 +0.3 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.4 +0.5 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 +1.0 N.D. -0.8
growth on ricinoleic acid YNB Glucose N.D. +0.6 -0.6 N.D. -0.0
growth on oleic acid YNB Glucose N.D. -0.2 -0.4 N.D. +0.6
growth on ypd YPD N.D. -0.1 +0.7 N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 -0.4 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 +0.1 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +1.1 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 +0.4 N.D. +1.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.6 N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 +0.5 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +1.3 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.8 N.D. +0.4
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