Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10598 and 10599 are separated by 345 nucleotides10599 and 10600 are separated by 1811 nucleotides10600 and 10601 are separated by 73 nucleotides10601 and 10602 are separated by 2535 nucleotides mRNA_2230: 10598 - HMMPfam-Protein kinase domain-PF00069,ProSitePatterns-Serine/Threonine protein kinases active-site signature.-PS00108,ProSiteProfiles-Protein kinase domain profile.-PS50011,SMART-Serine/Threonine protein kinases, catalytic domain-SM00220,SUPERFAMILY--SSF56112, at 419,903 to 421,996 10598 mRNA_2231: 10599 - Hypothetical Protein, at 422,342 to 422,815 10599 mRNA_2232: 10600 - Hypothetical Protein, at 424,627 to 425,038 10600 mRNA_2233: 10601 - Hypothetical Protein, at 425,112 to 425,465 10601 mRNA_2234: 10602 - K01626 E2.5.1.54, aroF, aroG, aroH 3-deoxy-7-phosphoheptulonate synthase, at 428,001 to 429,735 10602
Group Condition mRNA_2230 mRNA_2231 mRNA_2232 mRNA_2233 mRNA_2234
10598 10599 10600 10601 10602
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.0 N.D. -0.8 -2.1 N.D.
growth on ricinoleic acid YNB Glucose -0.6 N.D. -0.0 -0.9 N.D.
auxotrophy YNB Glucose 75mM Methionine -1.2 N.D. +0.6 -1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.0 N.D. -1.6 -0.8 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. +0.1 -1.1 N.D.
growth on ypd YPD +0.7 N.D. -0.6 -1.2 N.D.
growth on oleic acid YNB Oleic Acid -0.5 N.D. -0.1 -0.4 N.D.
auxotrophy YNB Glucose -1.1 N.D. +0.2 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. -0.7 +0.9 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. +0.3 -1.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. +0.5 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.4 +0.3 N.D.
auxotrophy Dropout Complete -0.1 N.D. -0.1 +0.7 N.D.
growth on oleic acid YNB Glucose -0.4 N.D. +0.6 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.1 N.D. -0.3 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.6 N.D. +0.5 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. +0.5 +0.6 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +1.2 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.5 N.D. -0.2 +1.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.8 N.D. +0.4 +0.6 N.D.
ypd buoyancy 40 hrs Low Buoyancy +1.3 N.D. -0.0 +1.3 N.D.
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