Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10600 and 10601 are separated by 73 nucleotides10601 and 10602 are separated by 2535 nucleotides10602 and 10603 are separated by 656 nucleotides10603 and 10604 are separated by 105 nucleotides mRNA_2232: 10600 - Hypothetical Protein, at 424,627 to 425,038 10600 mRNA_2233: 10601 - Hypothetical Protein, at 425,112 to 425,465 10601 mRNA_2234: 10602 - K01626 E2.5.1.54, aroF, aroG, aroH 3-deoxy-7-phosphoheptulonate synthase, at 428,001 to 429,735 10602 mRNA_2235: 10603 - K11843 USP14, UBP6 ubiquitin carboxyl-terminal hydrolase 14, at 430,392 to 432,550 10603 mRNA_2236: 10604 - KOG3098 Uncharacterized conserved protein, at 432,656 to 434,707 10604
Group Condition mRNA_2232 mRNA_2233 mRNA_2234 mRNA_2235 mRNA_2236
10600 10601 10602 10603 10604
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 -2.1 N.D. -0.6 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.6 -0.8 N.D. -0.6 -0.1
growth on ypd YPD -0.6 -1.2 N.D. -0.7 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.0 N.D. -2.0 -0.4
growth on ricinoleic acid YNB Glucose -0.0 -0.9 N.D. -0.4 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 +0.9 N.D. -1.3 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -1.2 N.D. -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.0 N.D. -0.3 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -1.1 N.D. +0.6 -0.7
growth on oleic acid YNB Oleic Acid -0.1 -0.4 N.D. +0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.3 N.D. -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.6 -1.0 N.D. +0.7 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.5 +0.6 N.D. -0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 +1.4 N.D. -0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.4 N.D. +0.8 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.6 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 +1.3 N.D. -0.1 -0.3
growth on oleic acid YNB Glucose +0.6 +0.4 N.D. +0.3 -0.2
auxotrophy Dropout Complete -0.1 +0.7 N.D. +0.7 -0.1
auxotrophy YNB Glucose +0.2 +0.3 N.D. +0.7 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +1.2 -0.4 N.D. -0.3 +1.2
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