Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10601 and 10602 are separated by 2535 nucleotides10602 and 10603 are separated by 656 nucleotides10603 and 10604 are separated by 105 nucleotides10604 and 10605 are separated by 577 nucleotides mRNA_2233: 10601 - Hypothetical Protein, at 425,112 to 425,465 10601 mRNA_2234: 10602 - K01626 E2.5.1.54, aroF, aroG, aroH 3-deoxy-7-phosphoheptulonate synthase, at 428,001 to 429,735 10602 mRNA_2235: 10603 - K11843 USP14, UBP6 ubiquitin carboxyl-terminal hydrolase 14, at 430,392 to 432,550 10603 mRNA_2236: 10604 - KOG3098 Uncharacterized conserved protein, at 432,656 to 434,707 10604 mRNA_2237: 10605 - KOG1794 N-Acetylglucosamine kinase, at 435,285 to 436,613 10605
Group Condition mRNA_2233 mRNA_2234 mRNA_2235 mRNA_2236 mRNA_2237
10601 10602 10603 10604 10605
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -2.1 N.D. -0.6 -0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. -2.0 -0.4 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -1.2 N.D. -0.4 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.8 N.D. -0.6 -0.1 -0.2
growth on ypd YPD -1.2 N.D. -0.7 -0.2 +0.4
growth on ricinoleic acid YNB Glucose -0.9 N.D. -0.4 -0.4 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.9 N.D. -1.3 -0.7 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.3 -0.5 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -1.1 N.D. +0.6 -0.7 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.1 +0.1 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. -0.3 +1.2 -0.5
growth on oleic acid YNB Oleic Acid -0.4 N.D. +0.4 -0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine -1.0 N.D. +0.7 +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.6 N.D. -0.1 -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. +0.8 -0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy +1.3 N.D. -0.1 -0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.6 N.D. -0.3 -0.2 +0.6
auxotrophy YNB Glucose +0.3 N.D. +0.7 +0.1 -0.3
growth on oleic acid YNB Glucose +0.4 N.D. +0.3 -0.2 +0.5
ypd buoyancy 40 hrs High Buoyancy +1.4 N.D. -0.3 -0.1 +0.1
auxotrophy Dropout Complete +0.7 N.D. +0.7 -0.1 -0.1
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