Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10602 and 10603 are separated by 656 nucleotides10603 and 10604 are separated by 105 nucleotides10604 and 10605 are separated by 577 nucleotides10605 and 10606 are separated by 137 nucleotides mRNA_2234: 10602 - K01626 E2.5.1.54, aroF, aroG, aroH 3-deoxy-7-phosphoheptulonate synthase, at 428,001 to 429,735 10602 mRNA_2235: 10603 - K11843 USP14, UBP6 ubiquitin carboxyl-terminal hydrolase 14, at 430,392 to 432,550 10603 mRNA_2236: 10604 - KOG3098 Uncharacterized conserved protein, at 432,656 to 434,707 10604 mRNA_2237: 10605 - KOG1794 N-Acetylglucosamine kinase, at 435,285 to 436,613 10605 mRNA_2238: 10606 - K20813 TDG thymine-DNA glycosylase, at 436,751 to 438,235 10606
Group Condition mRNA_2234 mRNA_2235 mRNA_2236 mRNA_2237 mRNA_2238
10602 10603 10604 10605 10606
facs on lipid accumulation Sorted No BODIPY Gate N.D. -2.0 -0.4 -0.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.3 -0.7 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.6 -0.3 -0.4 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 -0.5 -0.4 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 -0.2 -0.2 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.3 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.1 -0.9 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.6 -0.1 -0.2 +0.2
growth on ricinoleic acid YNB Glucose N.D. -0.4 -0.4 +0.3 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 -0.2 +0.6 -0.5
growth on ypd YPD N.D. -0.7 -0.2 +0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.1 +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.1 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.6 -0.7 -0.0 +0.1
growth on oleic acid YNB Glucose N.D. +0.3 -0.2 +0.5 -0.2
auxotrophy YNB Glucose N.D. +0.7 +0.1 -0.3 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +1.2 -0.5 +0.2
growth on oleic acid YNB Oleic Acid N.D. +0.4 -0.2 +0.4 +0.2
auxotrophy Dropout Complete N.D. +0.7 -0.1 -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.8 -0.1 +0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.7 +0.2 +0.3 +0.3
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