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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10605 and 10606 are separated by 137 nucleotides
10606 and 10607 are separated by 94 nucleotides
10607 and 10608 are separated by 400 nucleotides
10608 and 10609 are separated by 492 nucleotides
mRNA_2237: 10605 - KOG1794 N-Acetylglucosamine kinase, at 435,285 to 436,613
10605
mRNA_2238: 10606 - K20813 TDG thymine-DNA glycosylase, at 436,751 to 438,235
10606
mRNA_2239: 10607 - K18163 NDUFAF6 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 6, at 438,330 to 439,601
10607
mRNA_2240: 10608 - K13291 TUT terminal uridylyltransferase, at 440,002 to 443,977
10608
mRNA_2241: 10609 - K12572 PAN3 PAB-dependent poly(A)-specific ribonuclease subunit 3, at 444,470 to 447,596
10609
Group
Condition
mRNA
_2237
mRNA
_2238
mRNA
_2239
mRNA
_2240
mRNA
_2241
10605
10606
10607
10608
10609
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.2
-0.4
-3.3
-1.3
+0.7
facs on lipid accumulation
Sorted High BODIPY Gate
+0.6
-0.5
-1.1
-0.2
-0.5
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.0
+0.1
-2.8
-0.6
+1.5
ypd buoyancy
40 hrs Low Buoyancy
-0.3
-0.1
-0.5
-0.3
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
-0.4
-0.1
-1.3
+0.2
+0.1
auxotrophy
YNB Glucose
-0.3
-0.0
-0.7
-0.6
+0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.9
+0.2
+0.5
-0.7
+0.1
ypd buoyancy
40 hrs High Buoyancy
+0.1
+0.2
-0.4
-0.3
-0.1
growth on ricinoleic acid
YNB Glucose
+0.3
-0.0
-1.0
-0.1
+0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
-0.4
-0.6
+0.4
+0.5
ypd buoyancy
40 hrs No Buoyancy Separation
-0.5
+0.2
+0.4
-0.2
-0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.4
-0.4
-0.1
+0.1
+0.4
auxotrophy
YNB Glucose 75mM Arginine
+0.0
+0.3
-0.5
-0.4
+0.3
auxotrophy
YNB Glucose 75mM Methionine
+0.3
+0.3
-0.4
-0.6
+0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.4
+0.2
-0.6
-0.4
+1.1
ypd buoyancy
40 hrs Median Buoyancy
-0.2
+0.2
-0.1
+0.0
+0.1
auxotrophy
Dropout Complete
-0.1
+0.3
-0.3
-0.4
+0.6
growth on oleic acid
YNB Glucose
+0.5
-0.2
-0.0
+0.1
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
+0.2
-0.4
+0.4
+0.6
growth on ypd
YPD
+0.4
+0.0
+0.4
-0.2
+0.0
growth on oleic acid
YNB Oleic Acid
+0.4
+0.2
-0.2
-0.1
+0.5
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