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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10606 and 10607 are separated by 94 nucleotides
10607 and 10608 are separated by 400 nucleotides
10608 and 10609 are separated by 492 nucleotides
10609 and 10610 are separated by 466 nucleotides
mRNA_2238: 10606 - K20813 TDG thymine-DNA glycosylase, at 436,751 to 438,235
10606
mRNA_2239: 10607 - K18163 NDUFAF6 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 6, at 438,330 to 439,601
10607
mRNA_2240: 10608 - K13291 TUT terminal uridylyltransferase, at 440,002 to 443,977
10608
mRNA_2241: 10609 - K12572 PAN3 PAB-dependent poly(A)-specific ribonuclease subunit 3, at 444,470 to 447,596
10609
mRNA_2242: 10610 - Hypothetical Protein, at 448,063 to 449,685
10610
Group
Condition
mRNA
_2238
mRNA
_2239
mRNA
_2240
mRNA
_2241
mRNA
_2242
10606
10607
10608
10609
10610
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.4
-3.3
-1.3
+0.7
-0.4
facs on lipid accumulation
Sorted High BODIPY Gate
-0.5
-1.1
-0.2
-0.5
-0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
-2.8
-0.6
+1.5
-0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.1
-0.5
-0.3
-0.3
-0.0
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
-1.3
+0.2
+0.1
+0.1
auxotrophy
YNB Glucose
-0.0
-0.7
-0.6
+0.3
+0.0
growth on ricinoleic acid
YNB Glucose
-0.0
-1.0
-0.1
+0.4
-0.0
ypd buoyancy
40 hrs High Buoyancy
+0.2
-0.4
-0.3
-0.1
+0.1
auxotrophy
YNB Glucose 75mM Methionine
+0.3
-0.4
-0.6
+0.3
-0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.3
-0.5
-0.4
+0.3
-0.1
auxotrophy
Dropout Complete
+0.3
-0.3
-0.4
+0.6
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
+0.5
-0.7
+0.1
+0.1
growth on oleic acid
YNB Glucose
-0.2
-0.0
+0.1
+0.1
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.4
-0.1
+0.1
+0.4
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
-0.6
+0.4
+0.5
+0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
-0.6
-0.4
+1.1
-0.2
growth on ypd
YPD
+0.0
+0.4
-0.2
+0.0
-0.0
ypd buoyancy
40 hrs Median Buoyancy
+0.2
-0.1
+0.0
+0.1
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.2
+0.4
-0.2
-0.3
+0.3
growth on oleic acid
YNB Oleic Acid
+0.2
-0.2
-0.1
+0.5
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.2
-0.4
+0.4
+0.6
+0.1
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