Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10606 and 10607 are separated by 94 nucleotides10607 and 10608 are separated by 400 nucleotides10608 and 10609 are separated by 492 nucleotides10609 and 10610 are separated by 466 nucleotides mRNA_2238: 10606 - K20813 TDG thymine-DNA glycosylase, at 436,751 to 438,235 10606 mRNA_2239: 10607 - K18163 NDUFAF6 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 6, at 438,330 to 439,601 10607 mRNA_2240: 10608 - K13291 TUT terminal uridylyltransferase, at 440,002 to 443,977 10608 mRNA_2241: 10609 - K12572 PAN3 PAB-dependent poly(A)-specific ribonuclease subunit 3, at 444,470 to 447,596 10609 mRNA_2242: 10610 - Hypothetical Protein, at 448,063 to 449,685 10610
Group Condition mRNA_2238 mRNA_2239 mRNA_2240 mRNA_2241 mRNA_2242
10606 10607 10608 10609 10610
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -3.3 -1.3 +0.7 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -1.1 -0.2 -0.5 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -2.8 -0.6 +1.5 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.5 -0.3 -0.3 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -1.3 +0.2 +0.1 +0.1
auxotrophy YNB Glucose -0.0 -0.7 -0.6 +0.3 +0.0
growth on ricinoleic acid YNB Glucose -0.0 -1.0 -0.1 +0.4 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.4 -0.3 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.4 -0.6 +0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.5 -0.4 +0.3 -0.1
auxotrophy Dropout Complete +0.3 -0.3 -0.4 +0.6 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.5 -0.7 +0.1 +0.1
growth on oleic acid YNB Glucose -0.2 -0.0 +0.1 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.1 +0.1 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.6 +0.4 +0.5 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.6 -0.4 +1.1 -0.2
growth on ypd YPD +0.0 +0.4 -0.2 +0.0 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 +0.0 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.4 -0.2 -0.3 +0.3
growth on oleic acid YNB Oleic Acid +0.2 -0.2 -0.1 +0.5 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.4 +0.4 +0.6 +0.1
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