Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10611 and 10612 are separated by 803 nucleotides10612 and 10613 are separated by 943 nucleotides10613 and 10614 are separated by 362 nucleotides10614 and 10615 are separated by 194 nucleotides mRNA_2243: 10611 - SUPERFAMILY--SSF52266, at 450,168 to 452,098 10611 mRNA_2244: 10612 - KOG4120 G/T mismatch-specific thymine DNA glycosylase, at 452,902 to 454,246 10612 mRNA_2245: 10613 - K00134 GAPDH, gapA glyceraldehyde 3-phosphate dehydrogenase, at 455,190 to 456,884 10613 mRNA_2246: 10614 - K00026 MDH2 malate dehydrogenase, at 457,247 to 458,914 10614 mRNA_2247: 10615 - Hypothetical Protein, at 459,109 to 460,029 10615
Group Condition mRNA_2243 mRNA_2244 mRNA_2245 mRNA_2246 mRNA_2247
10611 10612 10613 10614 10615
auxotrophy YNB Glucose -0.2 -0.7 N.D. -1.1 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.2 N.D. -1.5 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 N.D. -1.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.7 N.D. -0.8 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 N.D. -1.0 +0.0
growth on oleic acid YNB Glucose -0.1 +0.0 N.D. -1.0 +0.0
growth on oleic acid YNB Oleic Acid -0.1 -0.4 N.D. -0.6 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.9 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.6 N.D. -0.1 -0.1
auxotrophy Dropout Complete -0.1 -0.1 N.D. -0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -1.3 N.D. -0.1 +0.6
growth on ricinoleic acid YNB Glucose -0.1 +0.9 N.D. -1.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.1 N.D. -0.5 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.1 N.D. -0.3 -0.2
growth on ypd YPD +0.6 -0.5 N.D. -0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.0 N.D. +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.5 N.D. +0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 N.D. +0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.9 N.D. -0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.6 N.D. +0.0 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +1.3 N.D. -0.5 +0.1
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