Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10612 and 10613 are separated by 943 nucleotides10613 and 10614 are separated by 362 nucleotides10614 and 10615 are separated by 194 nucleotides10615 and 10616 are separated by 351 nucleotides mRNA_2244: 10612 - KOG4120 G/T mismatch-specific thymine DNA glycosylase, at 452,902 to 454,246 10612 mRNA_2245: 10613 - K00134 GAPDH, gapA glyceraldehyde 3-phosphate dehydrogenase, at 455,190 to 456,884 10613 mRNA_2246: 10614 - K00026 MDH2 malate dehydrogenase, at 457,247 to 458,914 10614 mRNA_2247: 10615 - Hypothetical Protein, at 459,109 to 460,029 10615 mRNA_2248: 10616 - K01338 lon ATP-dependent Lon protease, at 460,381 to 463,216 10616
Group Condition mRNA_2244 mRNA_2245 mRNA_2246 mRNA_2247 mRNA_2248
10612 10613 10614 10615 10616
auxotrophy YNB Glucose -0.7 N.D. -1.1 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -1.0 +0.0 -0.6
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. -1.5 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -1.2 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 N.D. -0.1 -0.1 -0.7
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. -0.8 -0.3 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 N.D. -0.1 +0.6 -0.3
growth on oleic acid YNB Oleic Acid -0.4 N.D. -0.6 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 N.D. -0.1 +0.2 -0.3
growth on ypd YPD -0.5 N.D. -0.2 -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.5 +0.1 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. -0.3 -0.2 -0.1
auxotrophy Dropout Complete -0.1 N.D. -0.4 -0.1 -0.0
growth on oleic acid YNB Glucose +0.0 N.D. -1.0 +0.0 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. +0.4 +0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 N.D. +0.0 -0.3 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.0 +0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.0 +0.2 -0.0
growth on ricinoleic acid YNB Glucose +0.9 N.D. -1.3 -0.1 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 N.D. -0.2 -0.2 +1.0
growth on ricinoleic acid YNB Methylricinoleic Acid +1.3 N.D. -0.5 +0.1 +0.9
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