Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10617 and 10618 are separated by 151 nucleotides10618 and 10619 are separated by 403 nucleotides10619 and 10620 are separated by 262 nucleotides10620 and 10621 are separated by 264 nucleotides mRNA_2249: 10617 - K10753 ASF1 histone chaperone ASF1, at 463,565 to 464,577 10617 mRNA_2250: 10618 - HMMPfam-Apolipoprotein O-PF09769, at 464,729 to 465,798 10618 mRNA_2251: 10619 - KOG3313 Molecular chaperone Prefoldin, subunit 3, at 466,202 to 467,815 10619 mRNA_2252: 10620 - HMMPfam-Signal recognition particle 9 kDa protein (SRP9)-PF05486,SUPERFAMILY--SSF54762, at 468,078 to 468,584 10620 mRNA_2253: 10621 - HMMPfam-Staphylococcal nuclease homologue-PF00565,ProSiteProfiles-Thermonuclease domain profile.-PS50830,SMART-Staphylococcal nuclease homologues-SM00318,SUPERFAMILY--SSF50199, at 468,849 to 469,968 10621
Group Condition mRNA_2249 mRNA_2250 mRNA_2251 mRNA_2252 mRNA_2253
10617 10618 10619 10620 10621
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -1.5 N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 -1.2 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 -0.6 N.D. -0.2
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.6 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 -1.1 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.4 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.0 N.D. -0.3
auxotrophy YNB Glucose N.D. -0.0 -0.4 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.2 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -0.2 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.6 +0.5 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.2 N.D. +0.4
auxotrophy Dropout Complete N.D. +0.2 -0.1 N.D. +0.2
growth on oleic acid YNB Glucose N.D. +0.1 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.5 N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. -0.4 +0.8 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.7 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 +0.6 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.7 +0.9 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.2 N.D. +0.1
growth on ypd YPD N.D. -0.0 +0.6 N.D. +0.4
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