Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10619 and 10620 are separated by 262 nucleotides10620 and 10621 are separated by 264 nucleotides10621 and 10622 are separated by 227 nucleotides10622 and 10623 are separated by 427 nucleotides mRNA_2251: 10619 - KOG3313 Molecular chaperone Prefoldin, subunit 3, at 466,202 to 467,815 10619 mRNA_2252: 10620 - HMMPfam-Signal recognition particle 9 kDa protein (SRP9)-PF05486,SUPERFAMILY--SSF54762, at 468,078 to 468,584 10620 mRNA_2253: 10621 - HMMPfam-Staphylococcal nuclease homologue-PF00565,ProSiteProfiles-Thermonuclease domain profile.-PS50830,SMART-Staphylococcal nuclease homologues-SM00318,SUPERFAMILY--SSF50199, at 468,849 to 469,968 10621 mRNA_2254: 10622 - K17822 DCUN1D1_2 DCN1-like protein 1/2, at 470,196 to 471,391 10622 mRNA_2255: 10623 - Hypothetical Protein, at 471,819 to 472,358 10623
Group Condition mRNA_2251 mRNA_2252 mRNA_2253 mRNA_2254 mRNA_2255
10619 10620 10621 10622 10623
ypd buoyancy 40 hrs No Buoyancy Separation -1.5 N.D. -0.5 +0.8 N.D.
growth on oleic acid YNB Oleic Acid -0.6 N.D. +0.1 -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.1 N.D. +0.2 -0.1 N.D.
auxotrophy Dropout Complete -0.1 N.D. +0.2 -1.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.2 N.D. +0.1 +0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. -0.2 -0.0 N.D.
auxotrophy YNB Glucose -0.4 N.D. +0.3 -0.7 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.4 -0.9 N.D.
growth on oleic acid YNB Glucose -0.1 N.D. +0.2 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.3 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. +0.2 -0.8 N.D.
growth on ricinoleic acid YNB Glucose +0.8 N.D. +0.0 -0.9 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. +0.2 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. +0.3 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 N.D. +0.5 -1.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. +0.0 +0.5 N.D.
growth on ypd YPD +0.6 N.D. +0.4 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.1 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.6 N.D. +0.1 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. -0.1 +0.6 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.7 N.D. -0.0 +0.5 N.D.
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