Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10621 and 10622 are separated by 227 nucleotides10622 and 10623 are separated by 427 nucleotides10623 and 10624 are separated by 152 nucleotides10624 and 10625 are separated by 303 nucleotides mRNA_2253: 10621 - HMMPfam-Staphylococcal nuclease homologue-PF00565,ProSiteProfiles-Thermonuclease domain profile.-PS50830,SMART-Staphylococcal nuclease homologues-SM00318,SUPERFAMILY--SSF50199, at 468,849 to 469,968 10621 mRNA_2254: 10622 - K17822 DCUN1D1_2 DCN1-like protein 1/2, at 470,196 to 471,391 10622 mRNA_2255: 10623 - Hypothetical Protein, at 471,819 to 472,358 10623 mRNA_2256: 10624 - K14293 KPNB1, IPO1 importin subunit beta-1, at 472,511 to 475,600 10624 mRNA_2257: 10625 - K12626 LSM7 U6 snRNA-associated Sm-like protein LSm7, at 475,904 to 476,538 10625
Group Condition mRNA_2253 mRNA_2254 mRNA_2255 mRNA_2256 mRNA_2257
10621 10622 10623 10624 10625
growth on ricinoleic acid YNB Glucose +0.0 -0.9 N.D. N.D. N.D.
auxotrophy Dropout Complete +0.2 -1.0 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -1.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.8 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.6 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.9 N.D. N.D. N.D.
growth on oleic acid YNB Glucose +0.2 -0.6 N.D. N.D. N.D.
auxotrophy YNB Glucose +0.3 -0.7 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.0 N.D. N.D. N.D.
growth on ypd YPD +0.4 -0.6 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.1 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.2 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.3 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.2 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.8 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.5 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.5 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.6 N.D. N.D. N.D.
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