Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10626 and 10627 are separated by 299 nucleotides10627 and 10628 are separated by 269 nucleotides10628 and 10629 are separated by 349 nucleotides10629 and 10630 are separated by 180 nucleotides mRNA_2258: 10626 - K04627 STE3 pheromone a factor receptor, at 476,602 to 477,940 10626 mRNA_2259: 10627 - K02933 RP-L6, MRPL6, rplF large subunit ribosomal protein L6, at 478,240 to 479,053 10627 mRNA_2260: 10628 - K03027 RPC40, POLR1C DNA-directed RNA polymerases I and III subunit RPAC1, at 479,323 to 480,652 10628 mRNA_2261: 10629 - K12670 WBP1 oligosaccharyltransferase complex subunit beta, at 481,002 to 482,394 10629 mRNA_2262: 10630 - KOG0899 Mitochondrial/chloroplast ribosomal protein S19, at 482,575 to 483,044 10630
Group Condition mRNA_2258 mRNA_2259 mRNA_2260 mRNA_2261 mRNA_2262
10626 10627 10628 10629 10630
growth on ypd YPD -0.2 N.D. N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. N.D. N.D.
growth on oleic acid YNB Glucose +0.0 N.D. N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. N.D. N.D.
auxotrophy Dropout Complete +0.1 N.D. N.D. N.D. N.D.
auxotrophy YNB Glucose +0.1 N.D. N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. N.D. N.D. N.D.
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