Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10632 and 10633 are separated by 287 nucleotides10633 and 10634 are separated by 89 nucleotides10634 and 10635 are separated by 101 nucleotides10635 and 10636 are separated by 81 nucleotides mRNA_2264: 10632 - Hypothetical Protein, at 485,589 to 485,780 10632 mRNA_2265: 10633 - K08869 ADCK, ABC1 aarF domain-containing kinase, at 486,068 to 487,753 10633 mRNA_2266: 10634 - Hypothetical Protein, at 487,843 to 488,421 10634 mRNA_2267: 10635 - K15110 SLC25A21, ODC solute carrier family 25 (mitochondrial 2-oxodicarboxylate transporter), member 21, at 488,523 to 490,039 10635 mRNA_2268: 10636 - K11215 STE12 transcription factor STE12, at 490,121 to 491,043 10636
Group Condition mRNA_2264 mRNA_2265 mRNA_2266 mRNA_2267 mRNA_2268
10632 10633 10634 10635 10636
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.8 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.5 -0.1
growth on ypd YPD N.D. N.D. N.D. -0.1 -0.5
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.5 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.0 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.2 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 -0.3
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.1 +0.1
auxotrophy YNB Glucose N.D. N.D. N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 -0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.1 +0.1
auxotrophy Dropout Complete N.D. N.D. N.D. +0.4 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.3 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 +0.2
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