Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10638 and 10639 overlap by 2 nucleotides10639 and 10640 are separated by 461 nucleotides10640 and 10641 are separated by 442 nucleotides10641 and 10642 are separated by 146 nucleotides mRNA_2270: 10638 - K19833 CLA4 serine/threonine-protein kinase CLA4, at 492,259 to 494,809 10638 mRNA_2271: 10639 - Hypothetical Protein, at 494,808 to 495,275 10639 mRNA_2272: 10640 - Hypothetical Protein, at 495,737 to 495,928 10640 mRNA_2273: 10641 - K14787 MRD1, RBM19 multiple RNA-binding domain-containing protein 1, at 496,371 to 499,098 10641 mRNA_2274: 10642 - KOG2352 Predicted spermine/spermidine synthase, at 499,245 to 500,264 10642
Group Condition mRNA_2270 mRNA_2271 mRNA_2272 mRNA_2273 mRNA_2274
10638 10639 10640 10641 10642
facs on lipid accumulation Sorted Low BODIPY Gate -3.5 +0.2 N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -1.5 +0.3 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.9 +0.0 N.D. N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.2 N.D. N.D. +0.6
ypd buoyancy 40 hrs High Buoyancy -0.7 -0.1 N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.2 N.D. N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.1 N.D. N.D. +0.1
growth on ypd YPD +0.5 +0.2 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.1 N.D. N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.1 N.D. N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate +1.0 +0.3 N.D. N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine +1.1 +0.1 N.D. N.D. +0.0
growth on oleic acid YNB Glucose +0.9 -0.2 N.D. N.D. +0.6
auxotrophy YNB Glucose +1.5 -0.3 N.D. N.D. +0.1
auxotrophy Dropout Complete +1.6 -0.3 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +1.3 +0.1 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose +1.6 -0.0 N.D. N.D. +0.0
growth on oleic acid YNB Oleic Acid +1.8 -0.2 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +2.5 +0.1 N.D. N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +3.3 +0.0 N.D. N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +3.2 +0.4 N.D. N.D. +0.6
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