Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10641 and 10642 are separated by 146 nucleotides10642 and 10643 are separated by 332 nucleotides10643 and 10644 are separated by 298 nucleotides10644 and 10645 are separated by 216 nucleotides mRNA_2273: 10641 - K14787 MRD1, RBM19 multiple RNA-binding domain-containing protein 1, at 496,371 to 499,098 10641 mRNA_2274: 10642 - KOG2352 Predicted spermine/spermidine synthase, at 499,245 to 500,264 10642 mRNA_2275: 10643 - HMMPfam-MAPEG family-PF01124,SUPERFAMILY--SSF161084, at 500,597 to 501,422 10643 mRNA_2276: 10644 - K02954 RP-S14, MRPS14, rpsN small subunit ribosomal protein S14, at 501,721 to 502,247 10644 mRNA_2277: 10645 - K13717 OTUD3 OTU domain-containing protein 3, at 502,464 to 504,545 10645
Group Condition mRNA_2273 mRNA_2274 mRNA_2275 mRNA_2276 mRNA_2277
10641 10642 10643 10644 10645
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.3 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 -0.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.3 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 -0.7 N.D. +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.0 -0.5 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 +0.4 N.D. -0.3
auxotrophy Dropout Complete N.D. +0.1 -0.4 N.D. +0.2
growth on ypd YPD N.D. -0.0 -0.3 N.D. +0.1
growth on oleic acid YNB Glucose N.D. +0.6 -0.5 N.D. -0.2
auxotrophy YNB Glucose N.D. +0.1 -0.3 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 +0.8 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.4 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. +0.4 -0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 +0.6 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.4 +0.1 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.6 -0.2 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.6 +0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.6 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 +0.5 N.D. +0.3
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