Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10643 and 10644 are separated by 298 nucleotides10644 and 10645 are separated by 216 nucleotides10645 and 10646 are separated by 163 nucleotides10646 and 10647 are separated by 291 nucleotides mRNA_2275: 10643 - HMMPfam-MAPEG family-PF01124,SUPERFAMILY--SSF161084, at 500,597 to 501,422 10643 mRNA_2276: 10644 - K02954 RP-S14, MRPS14, rpsN small subunit ribosomal protein S14, at 501,721 to 502,247 10644 mRNA_2277: 10645 - K13717 OTUD3 OTU domain-containing protein 3, at 502,464 to 504,545 10645 mRNA_2278: 10646 - KOG1118 Lysophosphatidic acid acyltransferase endophilin/SH3GL, involved in synaptic vesicle formation, at 504,709 to 506,668 10646 mRNA_2279: 10647 - K16369 CHO2 phosphatidylethanolamine N-methyltransferase, at 506,960 to 510,896 10647
Group Condition mRNA_2275 mRNA_2276 mRNA_2277 mRNA_2278 mRNA_2279
10643 10644 10645 10646 10647
growth on oleic acid YNB Glucose -0.5 N.D. -0.2 -0.3 -1.7
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. -0.2 -0.5 -1.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. +0.2 +0.1 -2.2
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. +0.2 +0.2 -1.9
auxotrophy Dropout Complete -0.4 N.D. +0.2 +0.2 -2.2
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.1 +0.0 -1.9
growth on ricinoleic acid YNB Glucose -0.5 N.D. +0.2 +0.1 -1.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. -0.3 -0.2 -1.6
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.1 -0.1 -1.2
auxotrophy YNB Glucose -0.3 N.D. +0.1 +0.2 -1.7
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.1 -0.4 -1.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. -0.4 +0.2 -1.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.2 +0.4 -1.9
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 N.D. -0.4 -0.4 -1.4
growth on ypd YPD -0.3 N.D. +0.1 -0.6 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. -0.2 -0.4 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. +0.3 -0.3 -0.9
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.0 +0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. +0.2 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.1 +0.5 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.0 +0.5 -0.1
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