Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10646 and 10647 are separated by 291 nucleotides10647 and 10648 are separated by 482 nucleotides10648 and 10649 are separated by 232 nucleotides10649 and 10650 are separated by 842 nucleotides mRNA_2278: 10646 - KOG1118 Lysophosphatidic acid acyltransferase endophilin/SH3GL, involved in synaptic vesicle formation, at 504,709 to 506,668 10646 mRNA_2279: 10647 - K16369 CHO2 phosphatidylethanolamine N-methyltransferase, at 506,960 to 510,896 10647 mRNA_2280: 10648 - HMMPfam-B-block binding subunit of TFIIIC-PF04182, at 511,379 to 518,850 10648 mRNA_2281: 10649 - K12190 VPS36, EAP45 ESCRT-II complex subunit VPS36, at 519,083 to 521,393 10649 mRNA_2282: 10650 - HMMPfam-Conidiation protein 6-PF10346, at 522,236 to 522,967 10650
Group Condition mRNA_2278 mRNA_2279 mRNA_2280 mRNA_2281 mRNA_2282
10646 10647 10648 10649 10650
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -1.7 N.D. -2.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -1.6 N.D. -2.4 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -1.1 N.D. -2.3 -1.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -1.2 N.D. -2.5 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -2.2 N.D. -1.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 -1.9 N.D. -1.7 +0.2
auxotrophy YNB Glucose +0.2 -1.7 N.D. -1.6 -0.0
auxotrophy Dropout Complete +0.2 -2.2 N.D. -1.0 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.2 N.D. -0.5 -1.7
auxotrophy YNB Glucose 75mM Arginine +0.2 -1.9 N.D. -0.8 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.9 N.D. -1.2 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -1.4 N.D. -0.5 +0.5
growth on ricinoleic acid YNB Glucose +0.1 -1.7 N.D. -0.3 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -1.9 N.D. -0.5 +0.3
growth on oleic acid YNB Glucose -0.3 -1.7 N.D. +0.4 +0.1
growth on ypd YPD -0.6 -0.0 N.D. +0.0 -0.5
growth on oleic acid YNB Oleic Acid -0.4 -1.1 N.D. +0.4 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.5 N.D. +0.2 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 N.D. +0.7 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.5 -0.1 N.D. +0.6 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.5 +0.0 N.D. +1.3 -0.3
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