Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10662 and 10663 are separated by 123 nucleotides10663 and 10664 are separated by 77 nucleotides10664 and 10665 are separated by 689 nucleotides10665 and 10666 are separated by 208 nucleotides mRNA_2294: 10662 - KOG4131 Ngg1-interacting factor 3 protein NIF3L1, at 547,521 to 548,816 10662 mRNA_2295: 10663 - BLAST CsbD-like [Xanthophyllomyces dendrorhous], at 548,940 to 549,587 10663 mRNA_2296: 10664 - K05289 GAA1 glycosylphosphatidylinositol transamidase, at 549,665 to 551,990 10664 mRNA_2297: 10665 - HMMPfam-Acyltransferase-PF01553,SMART-Phosphate acyltransferases-SM00563,SUPERFAMILY--SSF69593, at 552,680 to 555,232 10665 mRNA_2298: 10666 - K15148 MED7 mediator of RNA polymerase II transcription subunit 7, at 555,441 to 556,485 10666
Group Condition mRNA_2294 mRNA_2295 mRNA_2296 mRNA_2297 mRNA_2298
10662 10663 10664 10665 10666
growth on oleic acid YNB Glucose +0.1 -0.3 N.D. -0.3 -0.5
growth on ypd YPD -0.4 -0.3 N.D. +0.2 -0.5
ypd buoyancy 40 hrs High Buoyancy -0.6 -0.1 N.D. +0.0 -0.2
auxotrophy YNB Glucose -0.2 -0.2 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 N.D. -0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.4 N.D. -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 N.D. -0.3 -0.2
growth on ricinoleic acid YNB Glucose +0.1 -0.1 N.D. -0.2 -0.2
growth on oleic acid YNB Oleic Acid +0.1 +0.0 N.D. -0.1 -0.5
auxotrophy Dropout Complete -0.0 -0.3 N.D. -0.2 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.2 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 N.D. -0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.3 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 +0.2 N.D. -0.3 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.3 N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 -0.1 N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 +0.1 N.D. +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.2 N.D. +0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.5 N.D. -0.1 +0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.7 N.D. -0.2 +0.4
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