Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10663 and 10664 are separated by 77 nucleotides10664 and 10665 are separated by 689 nucleotides10665 and 10666 are separated by 208 nucleotides10666 and 10667 are separated by 569 nucleotides mRNA_2295: 10663 - BLAST CsbD-like [Xanthophyllomyces dendrorhous], at 548,940 to 549,587 10663 mRNA_2296: 10664 - K05289 GAA1 glycosylphosphatidylinositol transamidase, at 549,665 to 551,990 10664 mRNA_2297: 10665 - HMMPfam-Acyltransferase-PF01553,SMART-Phosphate acyltransferases-SM00563,SUPERFAMILY--SSF69593, at 552,680 to 555,232 10665 mRNA_2298: 10666 - K15148 MED7 mediator of RNA polymerase II transcription subunit 7, at 555,441 to 556,485 10666 mRNA_2299: 10667 - Hypothetical Protein, at 557,055 to 559,337 10667
Group Condition mRNA_2295 mRNA_2296 mRNA_2297 mRNA_2298 mRNA_2299
10663 10664 10665 10666 10667
growth on oleic acid YNB Glucose -0.3 N.D. -0.3 -0.5 -0.1
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.1 -0.5 +0.0
growth on ypd YPD -0.3 N.D. +0.2 -0.5 +0.1
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.2 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.0 -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.3 -0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.3 -0.2 +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.2 +0.1 +0.4
auxotrophy YNB Glucose -0.2 N.D. -0.2 -0.2 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.3 -0.3 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.2 -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.1 -0.1 -0.1
auxotrophy Dropout Complete -0.3 N.D. -0.2 +0.0 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.2 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. -0.2 -0.1 +0.8
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.1 +0.1 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. -0.1 +0.5 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.7 N.D. -0.2 +0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.1 -0.2 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 -0.2 +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +0.1 +0.2 +0.5
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