Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10670 and 10671 are separated by 905 nucleotides10671 and 10672 are separated by 388 nucleotides10672 and 10673 are separated by 218 nucleotides10673 and 10674 are separated by 381 nucleotides mRNA_2302: 10670 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 563,015 to 564,806 10670 mRNA_2303: 10671 - Hypothetical Protein, at 565,712 to 566,657 10671 mRNA_2304: 10672 - Hypothetical Protein, at 567,046 to 568,484 10672 mRNA_2305: 10673 - K01082 cysQ, MET22, BPNT1 3'(2'), 5'-bisphosphate nucleotidase, at 568,703 to 570,355 10673 mRNA_2306: 10674 - HMMPfam-ATP synthase complex subunit h-PF10775, at 570,737 to 571,864 10674
Group Condition mRNA_2302 mRNA_2303 mRNA_2304 mRNA_2305 mRNA_2306
10670 10671 10672 10673 10674
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -2.0 +0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.4 -0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 -0.1 -0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.4 -0.2 -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.5 +0.1 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.1 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.5 -0.0 N.D. N.D.
auxotrophy YNB Glucose -0.4 +0.2 -0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.5 +0.3 -0.2 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.1 +0.1 -0.4 N.D. N.D.
growth on oleic acid YNB Glucose -0.1 +0.2 -0.4 N.D. N.D.
auxotrophy Dropout Complete -0.2 +0.4 -0.5 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.1 +0.2 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.1 +0.5 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.8 -0.3 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.6 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.4 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.5 +0.2 N.D. N.D.
growth on ypd YPD +0.4 +0.4 +0.2 N.D. N.D.
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