Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10673 and 10674 are separated by 381 nucleotides10674 and 10675 are separated by 426 nucleotides10675 and 10676 are separated by 217 nucleotides10676 and 10677 are separated by 368 nucleotides mRNA_2305: 10673 - K01082 cysQ, MET22, BPNT1 3'(2'), 5'-bisphosphate nucleotidase, at 568,703 to 570,355 10673 mRNA_2306: 10674 - HMMPfam-ATP synthase complex subunit h-PF10775, at 570,737 to 571,864 10674 mRNA_2307: 10675 - HMMPfam-Mitochondrial ATPase inhibitor, IATP-PF04568,SUPERFAMILY--SSF64602, at 572,291 to 573,066 10675 mRNA_2308: 10676 - ProSiteProfiles-VWFA domain profile.-PS50234,SUPERFAMILY--SSF53300, at 573,284 to 575,347 10676 mRNA_2309: 10677 - K10703 PHS1, PAS2 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase, at 575,716 to 577,026 10677
Group Condition mRNA_2305 mRNA_2306 mRNA_2307 mRNA_2308 mRNA_2309
10673 10674 10675 10676 10677
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 -0.4 N.D.
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.4 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 -0.2 N.D.
auxotrophy YNB Glucose N.D. N.D. +0.1 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.2 -0.2 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.0 -0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.4 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.3 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.2 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.1 N.D.
growth on ypd YPD N.D. N.D. +0.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 +0.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 +0.3 N.D.
growth on oleic acid YNB Glucose N.D. N.D. +0.2 +0.3 N.D.
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