Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10674 and 10675 are separated by 426 nucleotides10675 and 10676 are separated by 217 nucleotides10676 and 10677 are separated by 368 nucleotides10677 and 10678 are separated by 363 nucleotides mRNA_2306: 10674 - HMMPfam-ATP synthase complex subunit h-PF10775, at 570,737 to 571,864 10674 mRNA_2307: 10675 - HMMPfam-Mitochondrial ATPase inhibitor, IATP-PF04568,SUPERFAMILY--SSF64602, at 572,291 to 573,066 10675 mRNA_2308: 10676 - ProSiteProfiles-VWFA domain profile.-PS50234,SUPERFAMILY--SSF53300, at 573,284 to 575,347 10676 mRNA_2309: 10677 - K10703 PHS1, PAS2 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase, at 575,716 to 577,026 10677 mRNA_2310: 10678 - HMMPfam-AhpC/TSA antioxidant enzyme-PF13911,SUPERFAMILY--SSF52833, at 577,390 to 579,273 10678
Group Condition mRNA_2306 mRNA_2307 mRNA_2308 mRNA_2309 mRNA_2310
10674 10675 10676 10677 10678
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 +0.1 N.D. -0.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.2 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.2 N.D. -0.2
auxotrophy Dropout Complete N.D. -0.1 -0.3 N.D. -0.1
auxotrophy YNB Glucose N.D. +0.1 -0.4 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 -0.1 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.2 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 -0.4 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 -0.0 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 -0.4 N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.3 N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.3 N.D. +0.3
growth on ypd YPD N.D. +0.1 +0.1 N.D. -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.1 +0.3 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 +0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.4 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.1 N.D. +0.1
growth on oleic acid YNB Glucose N.D. +0.2 +0.3 N.D. +0.0
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