Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10675 and 10676 are separated by 217 nucleotides10676 and 10677 are separated by 368 nucleotides10677 and 10678 are separated by 363 nucleotides10678 and 10679 are separated by 737 nucleotides mRNA_2307: 10675 - HMMPfam-Mitochondrial ATPase inhibitor, IATP-PF04568,SUPERFAMILY--SSF64602, at 572,291 to 573,066 10675 mRNA_2308: 10676 - ProSiteProfiles-VWFA domain profile.-PS50234,SUPERFAMILY--SSF53300, at 573,284 to 575,347 10676 mRNA_2309: 10677 - K10703 PHS1, PAS2 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase, at 575,716 to 577,026 10677 mRNA_2310: 10678 - HMMPfam-AhpC/TSA antioxidant enzyme-PF13911,SUPERFAMILY--SSF52833, at 577,390 to 579,273 10678 mRNA_2311: 10679 - K20195 MON1 vacuolar fusion protein MON1, at 580,011 to 582,871 10679
Group Condition mRNA_2307 mRNA_2308 mRNA_2309 mRNA_2310 mRNA_2311
10675 10676 10677 10678 10679
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.1 N.D. -0.5 -1.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 N.D. -0.3 -1.2
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.2 N.D. -0.2 -0.9
auxotrophy YNB Glucose +0.1 -0.4 N.D. -0.2 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.4 N.D. +0.4 -0.7
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.3 N.D. +0.1 -0.6
auxotrophy Dropout Complete -0.1 -0.3 N.D. -0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.1 -0.3 N.D. +0.3 -0.6
growth on oleic acid YNB Oleic Acid +0.1 +0.3 N.D. -0.3 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.2 N.D. -0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.2 N.D. -0.3 +0.3
growth on oleic acid YNB Glucose +0.2 +0.3 N.D. +0.0 -0.8
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.4 N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.0 N.D. +0.1 +0.0
growth on ypd YPD +0.1 +0.1 N.D. -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.1 N.D. -0.1 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.4 N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.1 N.D. +0.4 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.2 N.D. +0.0 +1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.1 N.D. +0.1 +1.3
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