Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10676 and 10677 are separated by 368 nucleotides10677 and 10678 are separated by 363 nucleotides10678 and 10679 are separated by 737 nucleotides10679 and 10680 are separated by 389 nucleotides mRNA_2308: 10676 - ProSiteProfiles-VWFA domain profile.-PS50234,SUPERFAMILY--SSF53300, at 573,284 to 575,347 10676 mRNA_2309: 10677 - K10703 PHS1, PAS2 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase, at 575,716 to 577,026 10677 mRNA_2310: 10678 - HMMPfam-AhpC/TSA antioxidant enzyme-PF13911,SUPERFAMILY--SSF52833, at 577,390 to 579,273 10678 mRNA_2311: 10679 - K20195 MON1 vacuolar fusion protein MON1, at 580,011 to 582,871 10679 mRNA_2312: 10680 - K00275 pdxH, PNPO pyridoxamine 5'-phosphate oxidase, at 583,261 to 584,055 10680
Group Condition mRNA_2308 mRNA_2309 mRNA_2310 mRNA_2311 mRNA_2312
10676 10677 10678 10679 10680
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.3 -1.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.5 -1.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.2 -0.9 N.D.
auxotrophy YNB Glucose -0.4 N.D. -0.2 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. +0.1 -0.6 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. +0.4 -0.7 N.D.
growth on ricinoleic acid YNB Glucose -0.3 N.D. +0.3 -0.6 N.D.
auxotrophy Dropout Complete -0.3 N.D. -0.1 -0.2 N.D.
growth on oleic acid YNB Oleic Acid +0.3 N.D. -0.3 -0.6 N.D.
growth on oleic acid YNB Glucose +0.3 N.D. +0.0 -0.8 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.3 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.2 +0.2 N.D.
growth on ypd YPD +0.1 N.D. -0.3 -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. -0.3 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. +0.1 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. +0.1 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.1 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.4 +0.5 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.0 +1.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.1 +1.3 N.D.
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