Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10677 and 10678 are separated by 363 nucleotides10678 and 10679 are separated by 737 nucleotides10679 and 10680 are separated by 389 nucleotides10680 and 10681 are separated by 167 nucleotides mRNA_2309: 10677 - K10703 PHS1, PAS2 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase, at 575,716 to 577,026 10677 mRNA_2310: 10678 - HMMPfam-AhpC/TSA antioxidant enzyme-PF13911,SUPERFAMILY--SSF52833, at 577,390 to 579,273 10678 mRNA_2311: 10679 - K20195 MON1 vacuolar fusion protein MON1, at 580,011 to 582,871 10679 mRNA_2312: 10680 - K00275 pdxH, PNPO pyridoxamine 5'-phosphate oxidase, at 583,261 to 584,055 10680 mRNA_2313: 10681 - K13343 PEX14 peroxin-14, at 584,223 to 585,492 10681
Group Condition mRNA_2309 mRNA_2310 mRNA_2311 mRNA_2312 mRNA_2313
10677 10678 10679 10680 10681
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 -1.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 -1.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.9 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.6 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.0 -0.8 N.D. N.D.
auxotrophy YNB Glucose N.D. -0.2 -0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.6 N.D. N.D.
growth on ypd YPD N.D. -0.3 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.6 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 -0.7 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.1 -0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +0.2 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 +0.5 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +1.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +1.3 N.D. N.D.
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