Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10679 and 10680 are separated by 389 nucleotides10680 and 10681 are separated by 167 nucleotides10681 and 10682 are separated by 183 nucleotides10682 and 10683 are separated by 582 nucleotides mRNA_2311: 10679 - K20195 MON1 vacuolar fusion protein MON1, at 580,011 to 582,871 10679 mRNA_2312: 10680 - K00275 pdxH, PNPO pyridoxamine 5'-phosphate oxidase, at 583,261 to 584,055 10680 mRNA_2313: 10681 - K13343 PEX14 peroxin-14, at 584,223 to 585,492 10681 mRNA_2314: 10682 - BLAST Valine--tRNA ligase [Rhizoctonia solani], at 585,676 to 588,935 10682 mRNA_2315: 10683 - SUPERFAMILY--SSF160369, at 589,518 to 590,435 10683
Group Condition mRNA_2311 mRNA_2312 mRNA_2313 mRNA_2314 mRNA_2315
10679 10680 10681 10682 10683
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 N.D. N.D. -0.8 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -1.2 N.D. N.D. -0.6 N.D.
growth on oleic acid YNB Oleic Acid -0.6 N.D. N.D. -0.4 N.D.
growth on ricinoleic acid YNB Glucose -0.6 N.D. N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.9 N.D. N.D. +0.0 N.D.
growth on oleic acid YNB Glucose -0.8 N.D. N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.6 N.D. N.D. -0.1 N.D.
auxotrophy YNB Glucose -0.5 N.D. N.D. -0.1 N.D.
auxotrophy Dropout Complete -0.2 N.D. N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.0 N.D.
growth on ypd YPD -0.1 N.D. N.D. +0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.7 N.D. N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.3 N.D. N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +1.0 N.D. N.D. +0.2 N.D.
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